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Image Search Results
Journal: Methods in enzymology
Article Title: Methods for the expression, purification, and crystallization of histone deacetylase 6–inhibitor complexes
doi: 10.1016/bs.mie.2019.06.028
Figure Lengend Snippet: The purification scheme for zCD2 utilizes a HisTrap™ HP column, off-column digestion with 6 mg/ml TEV protease, a second HisTrap™ HP column for further purification, followed by a HiLoad™ Superdex™ 26/600 200 column.
Article Snippet: Equipment list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Autoclave (STERIS ® Healthcare) Innova ® 40 incubator shaker (New Brunswick) WPA CO8000 cell density meter (Biochrom) Sorvall LYNX 6000 centrifuge (Thermo Fisher Scientific) Revco ® ultima PLUS −80° freezer (Thermo Fisher Scientific) Vortex-Genie ® 2 (Scientific Industries) Sonicator (Qsonica) 5 mL HisTrap™ HP column (GE Healthcare) ÄKTA Prime Plus FPLC (GE Healthcare)
Techniques: Purification